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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Stability Indicating RP- HPLC - DAD Method for the Simultaneous Estimation of Hydrocortisone and Ketoconazole in Tablet Dosage form. |
| Authors Name: | Ramavath Mohanbabu naik , Syed Ahmed , Dr.D.Ramakrishna , Kambakam Venkatalakshmi |
| Unique Id: | IJSDR1901038 |
| Published In: | Volume 4 Issue 1, January-2019 |
| Abstract: | A new stability-indicating high performance liquid chromatographic method was developed and validated for the determination of Hydrocortisone and Ketoconazole in a tablet dosageform. The separation was achieved on a reverse phase C18 column (Agilent ODS) (250mm × 4.6 mm, 5 µ m) with mobile phase consisted of methanol:water (61:39 % v/v containing 0.2% HSA, pH 3.0 adjusted with Orthophosphoric acid) and the eluents were detected at 221nm. The retention time of Hydrocortisone and Ketoconazole was 6.0 min and 26.2 min respectively with the flow rate of 1mL.min-1. Drugs were subjected to stress conditions of acidic, basic, oxidation, photolytic, neutral and thermal degradation and considerable degradants were detected in all stress conditions. A total of 17 degradation products were observed and well separated from analyte peaks, hence the method could effectively employed as stability-indicating one. The response of linear was in the range 5-50 µg/mL for Hydrocortisone and 10 to 70 µg/mL for Ketoconazole, respectively. The relative standard deviation values for intra- and interday precision studies were 1.06 % and 1.5 % for Hydrocortisone and 0.46 % and 1.1% for Ketoconazole.Recoveries ranged in between 98-102% for both Hydrocortisone and Ketoconazole.. |
| Keywords: | RP-HPLC, Simultaneous, Hydrocortisone, Ketoconazole, stability-indicating |
| Cite Article: | "Stability Indicating RP- HPLC - DAD Method for the Simultaneous Estimation of Hydrocortisone and Ketoconazole in Tablet Dosage form.", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 1, page no.218 - 228, January-2019, Available :http://www.ijsdr.org/papers/IJSDR1901038.pdf |
| Downloads: | 000337077 |
| Publication Details: | Published Paper ID: IJSDR1901038 Registration ID:190034 Published In: Volume 4 Issue 1, January-2019 DOI (Digital Object Identifier): Page No: 218 - 228 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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