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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Screening, optimization and antimicrobial activity of Bromelain from Ananas comosus |
| Authors Name: | S.Bhagavathy , R.Gayathridevi , K.Pushya , J.Jeniffer |
| Unique Id: | IJSDR1908037 |
| Published In: | Volume 4 Issue 8, August-2019 |
| Abstract: | Bromelain is a major protease, isolated from pineapple (Ananas comosus). Bromelain is accumulated in the entire plant to different extent and properties depending on its source. In the present study, Bromelain was extracted from all parts of pineapple using sodium citrate buffer. Bromelain was filtered, centrifuged and used for further studies. After the determination of protease activity and protein content, the Core and Pulp extract of A.comosus was chosen using gelatin as the substrate. The samples were optimized on the basis of pH, temperature, Substrate concentration and etc. After optimization, the Bromelain was tested for antibacterial activity against bacterial pathogens. Among them, Pulp bromelain had maximum inhibition effect on Bacillus subtilis, Klebsiella pneumonia and Core bromelain had maximum inhibitory effect on Bacillus subtilis, Proteus vulgaris, Schigella flexneri and Escherichia coli and there was no inhibitory activity for the other tested pathogens. |
| Keywords: | Ananas comosus, Bromelain, pine apple, Antimicrobial activity, Optimization. |
| Cite Article: | "Screening, optimization and antimicrobial activity of Bromelain from Ananas comosus", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.4, Issue 8, page no.233 - 240, August-2019, Available :http://www.ijsdr.org/papers/IJSDR1908037.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR1908037 Registration ID:190913 Published In: Volume 4 Issue 8, August-2019 DOI (Digital Object Identifier): Page No: 233 - 240 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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