Development and validation of area under curve method for simultaneous estimation of clobetasol propionate and fusidic acid in cream

Mohapatra Deepali Pratapkumar , Dr. Alisha Patel

Clobetasol propionate, Fusidic acid, Area Under Curve, UVSpectroscopy method, Validation

A simple, specific, accurate and precise Area Under Curve spectrophotometric method was developed and validated for simultaneous estimation of Clobetasol propionate and Fusidic acid in cream. The linearity range was found to be 0.5- 2.5 μg/ml for Clobetasol propionate and 20-100 μg/ml for Fusidic acid. The co-relation co-efficient was found to be 0.9983, 0.9992 and 0.9989 ,0.9983 for Clobetasol propionate and Fusidic acid at λ1 233.00 -241.00 nm and λ2 220.00 – 228.00 nm respectively. The % recovery were found to be 99.71- 100.57 , 99.38- 100.45 and 99.65- 100.51 , 98.648-99.859 for Clobetasol propionate and Fusidic acid at λ1 233.00 -241.00 nm and λ2 220.00 – 228.00 nm respectively. Intraday precision were found to be 0.11-0.13, 0.19-0.24 and 0.29-0.31, 0.21-0.27 % RSD for Clobetasol propionate and Fusidic acid at λ1 233.00 -241.00 nm and λ2 220.00 – 228.00 nm and Interday precision were found to be within the range.

Published Paper   E-Certificate

"Development and validation of area under curve method for simultaneous estimation of clobetasol propionate and fusidic acid in cream", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.6, Issue 6, page no.47 - 62, June-2021, Available :https://ijsdr.org/papers/IJSDR2106008.pdf

Volume 6 Issue 6, June-2021

Pages : 47 - 62

Paper Reg. ID: IJSDR_193385

Published Paper Id: IJSDR2106008

Downloads: 000347045

Research Area: Engineering

Country: --, -, -

Published Paper PDF: https://ijsdr.org/papers/IJSDR2106008

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2106008

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition