A REVIEW ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Akshada Laxman Vitnor , Rajeshree Khandre

HPLC, Types, Application, Analytical method validation, pharmaceutical analysis

High performance liquid chromatography (HPLC) is an essential analytical tool in assessing drug product. HPLC methods should be able to separate, detect, and quantify the various drugs and drug related degradants that can form on storage or manufacturing, detect and quantify any drugs and drug-related impurities that may be introduced during synthesis. Today HPLC is widely applied for separations and purify cations in a variety of areas including pharmaceuticals, biotechnology, environmental, polymer and food industries. It is accomplished by injection of a small amount of liquid sample into a moving stream of liquid (called the mobile phase) that passes through a column packed with particles of the stationary phase. The separation of a mixture into its components depends on different degrees of retention of each component in the column. HPLC is just one type of liquid chromatography, meaning the mobile phase is a liquid. This review mainly focuses on the HPLC technique its principle, types, instrumentation and applications. Although several analytical techniques are available for the quantification of steroids, their analysis is challenging due to their low levels and complex matrices of the samples. The efficiency and quick separation of the HPLC combined with the sensitivity, selectivity, simplicity, and cost-efficiency of fluorescence, make HPLC coupled to fluorescence detection (HPLC-FLD) an ideal tool for routine measurement and detection of steroids

Published Paper   E-Certificate

"A REVIEW ON HIGH PERFORMANCE LIQUID CHROMATOGRAPHY", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.7, Issue 12, page no.556 - 563, December-2022, Available :https://ijsdr.org/papers/IJSDR2212086.pdf

Volume 7 Issue 12, December-2022

Pages : 556 - 563

Paper Reg. ID: IJSDR_203037

Published Paper Id: IJSDR2212086

Downloads: 000347296

Research Area: Pharmacy

Country: rahuri, Maharashtra, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2212086

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2212086

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition