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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Chromatography latest techniques |
| Authors Name: | D.Rama Brahma Reddy , K.Malleswari , Mallela Bhavani |
| Unique Id: | IJSDR2402026 |
| Published In: | Volume 9 Issue 2, February-2024 |
| Abstract: | High-Performance Liquid Chromatography (HPLC) is a type of column chromatography that is commonly used in biochemistry and analysis to separate, identify, and quantify active chemicals. HPLC is the most often used separation technology for detecting, separating, and quantifying the drug. HPLC technique development and validation serve critical roles in novel drug discovery, development, and manufacturing, as well as a variety of other human and animal investigations. This review discusses the many processes involved in developing and validating an HPLC technique. The creation of an HPLC technique is influenced by the chemical structure of the molecules, the synthetic pathway, solubility, polarity, pH and pKa values, and the activity of functional groups, among other factors. Accuracy, accuracy, specificity, linearity, range, limit of detection, the limit of quantification, robustness, and system suitability testing are all included in the validation of an HPLC technique according to ICH Guidelines. |
| Keywords: | Pressure Liquid Chromatography, Chromatography, Method validation, Method development. |
| Cite Article: | "Chromatography latest techniques", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.9, Issue 2, page no.176 - 183, February-2024, Available :http://www.ijsdr.org/papers/IJSDR2402026.pdf |
| Downloads: | 000338721 |
| Publication Details: | Published Paper ID: IJSDR2402026 Registration ID:209995 Published In: Volume 9 Issue 2, February-2024 DOI (Digital Object Identifier): Page No: 176 - 183 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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