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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Proximate Analysis and Estimation of Flavan-3-ol Compounds Present in Grape Seed Extract by HPLC Method |
| Authors Name: | Muthulakshmi C , Priya J , Mayilvaganan M , Purushothaman M |
| Unique Id: | IJSDR2404216 |
| Published In: | Volume 9 Issue 4, April-2024 |
| Abstract: | Grape seeds are produced as waste products of the winery and grape juice-making industries. The United States Food and Drug Administration (FDA) recognized grape seeds as safe food. Flavan-3ols are grouped under the polyphenols, which are present in various fruits naturally. The flavan-3-ol compounds present in the grape seeds have potential effects on several diseases, like endocrine disorders, cancer and diabetes mellitus. Proximate estimation is used for analyzing moisture, total fat, carbohydrate, total phenols and flavonoids. High Performance Liquid Chromatography (HPLC) is a widely used and convenient method that helps to separate compounds and helps to “identify compounds” using various detection systems. The separation was achieved using a C18 analytical column and isocratic elution was carried out. The mobile phase is made of acetonitrile-formic acid (15:85). A 275 nm wavelength was set for the detection. The grape seeds were extracted using acetone. |
| Keywords: | Grape seeds, flavan-3-ol, proximate analysis, HPLC, isocratic elution. |
| Cite Article: | "Proximate Analysis and Estimation of Flavan-3-ol Compounds Present in Grape Seed Extract by HPLC Method", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.9, Issue 4, page no.1473 - 1478, April-2024, Available :http://www.ijsdr.org/papers/IJSDR2404216.pdf |
| Downloads: | 000338172 |
| Publication Details: | Published Paper ID: IJSDR2404216 Registration ID:211135 Published In: Volume 9 Issue 4, April-2024 DOI (Digital Object Identifier): Page No: 1473 - 1478 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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