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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | In vitro multiplication of Maytenus emarginata. - A tree of the Thar Desert |
| Authors Name: | DR NARPAT SINGH DEORA |
| Unique Id: | IJSDR1707070 |
| Published In: | Volume 2 Issue 7, July-2017 |
| Abstract: | An in vitro method for cloning and mass multiplication of Maytenus emarginata, a highly drought resistant tree of the Indian Desert, has been developed. Shoot segments harvested from a "plus" tree (30-year-old) were cultured to produce multiple shoots (10-15 shoots/explant) on MS medium containing 0.1 mg/l IAA and 2.5 mg/l BAP. In vitro produced shoots were cut into segments and cultured on shoot proliferation medium supplimented with 1.0 mg/1 of BAP for further multiplication of the shoots. Isolated individual shoots were cultured on a filter paper bridge in half strength MS liquid medium containing 25 mg/l of IBA for 72 h in the dark at 28 + 2° C for induction of root. About 70-80 percent of shoots rooted. The treelets developed were hardened and transferred to pots. Thousands of plantlets can be obtained from a single explants. The protocol is highly reproducible and efficient. |
| Keywords: | Maytenus emarginata, Cloning, Tissue culture, Treelet, Regeneration |
| Cite Article: | "In vitro multiplication of Maytenus emarginata. - A tree of the Thar Desert", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.2, Issue 7, page no.417 - 419, July-2017, Available :http://www.ijsdr.org/papers/IJSDR1707070.pdf |
| Downloads: | 000337353 |
| Publication Details: | Published Paper ID: IJSDR1707070 Registration ID:207773 Published In: Volume 2 Issue 7, July-2017 DOI (Digital Object Identifier): Page No: 417 - 419 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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