DETECTION AND ANTIBIOGRAM OF EXTENDED SPECTRUM BETALACTAMASE (ESBL) PRODUCING ESCHERICHIA COLI ISOLATED FROM URINE SAMPLES AT GOVERMENT MEDICAL COLLEGE,KOTA RAJASTHAN
Dr. Bhupendra Kumar Mandawat
ESBLs, UTI, ESCHERICHIA COLI, CLSI, DDST, PCDDT, E-test ESBL STRIP
ABSTRACT: The spread of Extended Spectrum Beta-Lactamases (ESBLs) producing bacteria has become strikingly rapid worldwide, indicating that continuous monitoring systems and effective infection control measures are absolutely required. The therapeutic options for the infections which are caused by these organisms have also become increasingly limited. ESBLs producing strains have emerged as a significant challenge to counter with present antibiotics. BACKGROUND: Escherichia coli is the most common organism causing urinary tract infection (UTI). This organism has the ability to produce ESBLs which confer multiple drug resistance making urinary tract infection difficult to treat. OBJECTIVES: The aims of this study were to detect prevalence of ESBL production among E. coli isolates in urine samples and to detect their antibiotic susceptibility pattern at Govt. Medical College, Kota. This study demonstrate the importance of regular review of empirical antibiotic therapy for UTI in view of the evolving resistance of ESBL producing E. coli to commonly used antimicrobial agents. METHODOLOGY: The present study was conducted in the clinical microbiology laboratory of Govt. Medical College, Kota from period of 1st January, 2018 to 31th December 2018 to evaluate prevalence of ESBL production among E. coli isolates in urine samples and to detect their antibiotic susceptibility pattern. RESULT: A total of 500 consecutive, nonrepetitive E. coli isolates were studied. Antimicrobial susceptibility test was performed on Muller Hinton agar plates using Kirby Bauer disk diffusion method. ESBL detection was done for all isolates according to latest CLSI criteria. These isolates were confirmed for ESBL production by the double disc synergy test (DDST) and the phenotypic confirmatory disc diffusion test (PCDDT) and Twenty-five randomly selected isolates were further confirmed by the E-test ESBL strip randomly. Out of 500 E. coli isolate, 310(62%) were ESBL producers and 190(38%) were Non ESBL producers. Out of the 310 isolates which were tested,228(73.55%) were found to be ESBL producers by PCDDT,193 (62.26%) were found to be ESBL producers by DDST and 19(76%), were found to be ESBL producers by the E-test ESBL strip which showed a highly significant correlation with PCDDT. Conclusion: As results showed that there was a high prevalence of ESBL production in our setup so, it is essential to report the ESBL production along with the routine sensitivity reports, which will help the clinician in selection of proper antibiotics.
"DETECTION AND ANTIBIOGRAM OF EXTENDED SPECTRUM BETALACTAMASE (ESBL) PRODUCING ESCHERICHIA COLI ISOLATED FROM URINE SAMPLES AT GOVERMENT MEDICAL COLLEGE,KOTA RAJASTHAN", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.4, Issue 7, page no.272 - 279, July-2019, Available :https://ijsdr.org/papers/IJSDR1907047.pdf
Volume 4
Issue 7,
July-2019
Pages : 272 - 279
Paper Reg. ID: IJSDR_190798
Published Paper Id: IJSDR1907047
Downloads: 000347266
Research Area: Biological Science
Country: KOTA, RAJASTHAN, India
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
