Screening, optimization and antimicrobial activity of Bromelain from Ananas comosus

S.Bhagavathy , R.Gayathridevi , K.Pushya , J.Jeniffer

Ananas comosus, Bromelain, pine apple, Antimicrobial activity, Optimization.

Bromelain is a major protease, isolated from pineapple (Ananas comosus). Bromelain is accumulated in the entire plant to different extent and properties depending on its source. In the present study, Bromelain was extracted from all parts of pineapple using sodium citrate buffer. Bromelain was filtered, centrifuged and used for further studies. After the determination of protease activity and protein content, the Core and Pulp extract of A.comosus was chosen using gelatin as the substrate. The samples were optimized on the basis of pH, temperature, Substrate concentration and etc. After optimization, the Bromelain was tested for antibacterial activity against bacterial pathogens. Among them, Pulp bromelain had maximum inhibition effect on Bacillus subtilis, Klebsiella pneumonia and Core bromelain had maximum inhibitory effect on Bacillus subtilis, Proteus vulgaris, Schigella flexneri and Escherichia coli and there was no inhibitory activity for the other tested pathogens.

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"Screening, optimization and antimicrobial activity of Bromelain from Ananas comosus", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.4, Issue 8, page no.233 - 240, August-2019, Available :https://ijsdr.org/papers/IJSDR1908037.pdf

Volume 4 Issue 8, August-2019

Pages : 233 - 240

Paper Reg. ID: IJSDR_190913

Published Paper Id: IJSDR1908037

Downloads: 000347207

Research Area: Biological Science

Country: Chennai, Tamilnadu, Country

Published Paper PDF: https://ijsdr.org/papers/IJSDR1908037

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR1908037

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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