Development and Validation of RP-HPLC and UV-Spectrophotometric Absorptivity Method for Simultaneous Estimation of Cyclobenzaprine hydrochloride and Aceclofenac in Pharmaceutical dosage form

Annasaheb Sanjay Gaikwad , Monika Namdev Madibone , Jeevan R Rajguru , Prashant B Jadhav

Cyclobenzaprine hydrochloride and Aceclofenac, UV- Spectrophotometric, RP-HPLC Method.

The objective of present research is to develop a simple, sensitive, linear, precise and accurate RP-HPLC and UV-Spectrophotometric method for simultaneous estimation of Cyclobenzaprine hydrochloride and Aceclofenac in bulk and tablet formulation as developed and validated. UV-Spectrophotometric method Calibration plot were linear R2 =0.9996 over the concentration range 1-5µg/ml for Cyclobenzaprine hydrochloride, R²= 0.9995 for the Aceclofenac 13-65µg/ml. And Chromatographic conditions used are stationary phase Inertsil ODS column (250 × 4.6 mm×5µ) (5µm particle size) the mobile phase Methanol: 10mm KH2PO4 Buffer (Ph-3) (70:30) and flow rate was maintained 0.9ml/min, detection wavelength was 220nm. The retention times were 4.7min and 2.9 min for Cyclobenzaprine hydrochloride and Aceclofenac respectively. Calibration plot were linear R2 = 0.9996 over the concentration range 3-15µg/ml for Cyclobenzaprine hydrochloride, R2 = 0.9994 for the Aceclofenac 40-200µg/ml. No interference from any component of pharmaceutical dosage form was observed. The proposed method has been validated as per ICH guidelines, validation studies revealed that method id specific, rapid, reliable and reproducible. The developed method successfully employed for routine quality control analysis in the combined pharmaceutical dosage form.

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"Development and Validation of RP-HPLC and UV-Spectrophotometric Absorptivity Method for Simultaneous Estimation of Cyclobenzaprine hydrochloride and Aceclofenac in Pharmaceutical dosage form", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.5, Issue 4, page no.320 - 329, April-2020, Available :https://ijsdr.org/papers/IJSDR2004056.pdf

Volume 5 Issue 4, April-2020

Pages : 320 - 329

Paper Reg. ID: IJSDR_191659

Published Paper Id: IJSDR2004056

Downloads: 000347298

Research Area: Pharmacy

Country: AURANGABAD, Maharashtra, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2004056

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2004056

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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