Designing Media from Domestic Waste to Enhance Growth of Chlorococcum humicola and Its Application on Mosquito Control
Mynumathi Maragatham
, Sasikala Chenniyappan , Geetharamani Durairaj
Chlorococcum humicola, nutrient, media development, applications, larvicidal, Mosquito control, Domestic.
Objective: To design media from domestic waste with limited nitrogen and high of other nutrients (potassium, phosphorus, vitamins and trace elements) for enhance biomass and bioactive compounds of Chlorococcum humicola with potential application on mosquito control. Methodology: Microalgae Chlorococcum humicola was isolated from lake and cultured using BG11 media. Ingredients were made from domestic waste materials through the processes like collection, shade drying, mincing, cold extraction and sterilization by membrane filtration method. The items were BG11 media, BG11 with ingredients of designed media, and designed media with optimum concentration of ingredients inoculated with 5% of inoculum. Growth was monitored from 0th day to 25th day by the measurement of optical density, chlorophyll content and dry weight. Larvicidal evaluation were done by exposing 4-6 larva into 100ppm, 200ppm, 300ppm, 400ppm, 500 ppm concentrations of ethanolic algal extract of Chlorococcum humicola. Result and discussion: Chlorococcum humicoal was isolated from lake water and identified, the diverse parameters of growth was measured in three different media during the 0 to 25 days, gradual increase were showed in algae cells(optical density), chlorophyll content and final weight of algal biomass. Complete mortality of larva species were seen in 500ppm bestowed the efficacy of Chlorococcum humicola to kill mosquito species with small concentrations which was cultivated in the designed media compared to BG11 and BG11 with ingredients of designed media. From this study, with limited nitrogen source and optimum level of nutrients such as potassium, phosphorus, vitamins, trace elements from different domestic waste materials was highly supported the growth of Chlorococcum humicola and improved their efficacy for potential applications. Key words: Chlorococcum humicola, nutrient, media development, applications, larvicidal, Mosquito control, Domestic.
"Designing Media from Domestic Waste to Enhance Growth of Chlorococcum humicola and Its Application on Mosquito Control", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.5, Issue 5, page no.102 - 111, May-2020, Available :https://ijsdr.org/papers/IJSDR2005020.pdf
Volume 5
Issue 5,
May-2020
Pages : 102 - 111
Paper Reg. ID: IJSDR_191716
Published Paper Id: IJSDR2005020
Downloads: 000347209
Research Area: Biological Science
Country: COIMBATORE, TAMIL NADU, India
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
