Rp-hplc method development and validation for simultaneous estimation of lopinavir and ritonavir in bulk and tablet dosage form

Althaf.Shaik , Reshma.Shaik , Prachet.Pinnamaneni

Ritonavir,lopinavir,Method development, RP-HPLC.

A simple, Accurate, precise method was developed for the simultaneous estimation of the ritonavir and lopinavir in bulk and tablet dosage form.Chromatogram was run through schimadzu C18 (250 x 4.6 mm, 5m).Mobile phase containing Acetonitrile :phosphate Buffer taken in the ratio 60:40v/v was pumped through column at a flow rate of 1.2 ml/min. Buffer used in this method was 0.1% OPA buffer. Optimized wavelength selected was 226 nm. Retention time of ritonavir and lopinavir were found to be 6.428min and 7.325 min. %RSD of the ritonavir and lopinavir were and found to be 0.7 and 0.8 respectively. %Recovery was obtained as 100.16% and 98.96% for ritonavir and lopinavir.LOD, LOQ values obtained from regression equations of ritonavir and lopinavir were 4.08, 3.49 and 12.3, 10.5 respectively.Regression equation of ritonavir is y =13739x+11111, and y = 3713x+3510 of lopinavir.Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular quality control test in Industries.

Published Paper   E-Certificate

"Rp-hplc method development and validation for simultaneous estimation of lopinavir and ritonavir in bulk and tablet dosage form", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.5, Issue 9, page no.277 - 289, September-2020, Available :https://ijsdr.org/papers/IJSDR2009046.pdf

Volume 5 Issue 9, September-2020

Pages : 277 - 289

Paper Reg. ID: IJSDR_192463

Published Paper Id: IJSDR2009046

Downloads: 000347211

Research Area: Pharmacy

Country: Guntur, Andhra pradesh, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2009046

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2009046

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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