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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM SALIVA SAMPLE AND OPTIMIZATION OF EXTRACTED CELLULASE |
| Authors Name: | Ghanwat Kritika , Lahudkar Dnyaneshwari , |
| Unique Id: | IJSDR2205120 |
| Published In: | Volume 7 Issue 5, June-2022 |
| Abstract: | Microorganisms have a great potential in degradation of cellulose. Among which, bacteria have a major contribution. This study was conducted to isolate and characterize cellulose degrading bacteria which was collected from ‘Jaffrabadi’ breed of buffalo by morphological and biochemical analysis. The cellulose degrading bacteria were isolated, screened and purified on CMC agar plates. Screening resulted in selection of bacterial isolate producing clear zone when plates were flooded with Congo red and washed with NaCl, which confirmed the cellulose degradation. The morphological characterization showed the bacteria to be a Gram positive, motile, nonsporulating rod-shaped bacterium. The biochemical tests indicated that the bacteria belonged to the Cellulomonas spp. (according to Bergey’s Manual of Determinative Bacteriology). The bacterial isolate was then subjected to cellulase production, which was further optimized with various parameters like incubation time, temperature, pH and also with different carbon and nitrogen sources. |
| Keywords: | Jaffrabadi, degrading, characterize, CMC agar, Cellulomonas |
| Cite Article: | "ISOLATION AND CHARACTERIZATION OF CELLULASE PRODUCING BACTERIA FROM SALIVA SAMPLE AND OPTIMIZATION OF EXTRACTED CELLULASE", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 5, page no.675 - 679, June-2022, Available :http://www.ijsdr.org/papers/IJSDR2205120.pdf |
| Downloads: | 000337072 |
| Publication Details: | Published Paper ID: IJSDR2205120 Registration ID:200481 Published In: Volume 7 Issue 5, June-2022 DOI (Digital Object Identifier): Page No: 675 - 679 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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