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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | A Validated RP-HPLC Method for Analysis of Chloramphenicol Residue in Pasteurized Milk Sample in Bangladesh |
| Authors Name: | Hasib Khan Shomudro , Mithila Khatun , Sherejad Sanam |
| Unique Id: | IJSDR2209005 |
| Published In: | Volume 7 Issue 9, September-2022 |
| Abstract: | Abstract: The present study of this research was to develop a useful HPLC sensitive method to validate and determine the presence of trace amount of chloramphenicol in pasteurized milk. Method was developed by using Waters C18 (150×4.6, 5µm). The mobile phase was isocratic, composed of phosphate buffer pH 6.8 and acetonitrile (20:80), pumped at a flow rate of 1 mL/minute, and wavelength was 270 nm. The method was validated with deproteinized milk sample using Trichloro acetic acid and acetonitrile for system suitability, accuracy, linearity, precision study, robustness, limit of detection, and limit of quantification. Finally, determination of chloramphenicol of tested samples were observed. Trace amount of chloramphenicol in Bangladesh is rare. The findings of this research may aid in the investigation of the existence of trace amounts of chloramphenicol in pasteurized milk samples, as well as the fact that the presence of antibiotics in foods poses a serious hazard to health of human. From this study, we can define that chloramphenicol presence may not found, but if it was present in a minimal amount that may have an alarming issue but range was very minimal. |
| Keywords: | Key Words: Chloramphenicol, Pasteurized milk, RP-HPLC, Robustness, Mobile phase, Quantification. |
| Cite Article: | "A Validated RP-HPLC Method for Analysis of Chloramphenicol Residue in Pasteurized Milk Sample in Bangladesh", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 9, page no.33 - 40, September-2022, Available :http://www.ijsdr.org/papers/IJSDR2209005.pdf |
| Downloads: | 000337077 |
| Publication Details: | Published Paper ID: IJSDR2209005 Registration ID:201391 Published In: Volume 7 Issue 9, September-2022 DOI (Digital Object Identifier): Page No: 33 - 40 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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