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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | HYDROPHILLIC INTERACTION LIQUID CHROMATOGRAPHY AS A TOOL FOR QUANTIFICATION OF POLAR ANALYTES |
| Authors Name: | Ankita , Amardeep Ankalgi , Kamya Goyal , C.P.S Verma , Mahendra Singh Ashawat |
| Unique Id: | IJSDR2209047 |
| Published In: | Volume 7 Issue 9, September-2022 |
| Abstract: | : Hydrophilic Interaction liquid chromatography (HILIC) is a preferred seperation method for polar analyte as compare to reversed phase liquid chromatography. The HILIC methods were reported to be cost effetive, accurate , precise and robust especially for polar analytes such as glycans. The stationary phases mostly consist of different functional groups and dependng upon the column chemistry , it is further classified into neutral, charged, and zwitterion phases. HILIC mobile phases composition consist of high concentration of acetonitrile (60-97%) and a low concentration of water ( 3- 40) that make polar analytes to diffuse into surface of stationary phase for the interaction. The review article focued on basic principle, , HILIC stationary phases and its application. Small and large hydrophilic and genuinely polar granules, such as glycans, peptides and proteins, nucleosides, vitamins, phenols, fungicides, venoms, and a wide range of polychrome hydrophilic metabolites are separated using the HILIC system from different pharmaceutical products , food samples , water sample and biological matrices . |
| Keywords: | HILIC; Stationary phase; Column; ACN; Water Layer. |
| Cite Article: | "HYDROPHILLIC INTERACTION LIQUID CHROMATOGRAPHY AS A TOOL FOR QUANTIFICATION OF POLAR ANALYTES ", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.7, Issue 9, page no.300 - 304, September-2022, Available :http://www.ijsdr.org/papers/IJSDR2209047.pdf |
| Downloads: | 000337070 |
| Publication Details: | Published Paper ID: IJSDR2209047 Registration ID:201611 Published In: Volume 7 Issue 9, September-2022 DOI (Digital Object Identifier): Page No: 300 - 304 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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