INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016
open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15
Study of amylase producing bacteria isolated from vegetable market dumping ground
Authors Name:
Aniket Ramchandra Agrahari
, Sunil R. Jagiasi
Unique Id:
IJSDR2305382
Published In:
Volume 8 Issue 5, May-2023
Abstract:
Amylases are one of the most important industrial enzymes that hydrolyze starch molecules into fine products such as dextrin and maltose. Microbes were isolated and identified from vegetable waste dumping area. In recent years, interest in microbial production of the amylase has increased dramatically due to its wide use in the bakery, food, textile and the detergent industry. The purpose of this research study is to isolate indigenous amylase producing bacteria from vegetable waste dumping area. Samples collected from in an around Bhiwandi, district - Thane, Maharashtra. Randomly 19 isolates were selected based on variation in appearance for further studies. All isolates were tested for Quantitatively amylase production using DNSA. The potent isolate showing higher amylase activity of 144 µg/ml/min was identified as belonging to Bacillus thuringiensis by using 16s rRNA gene sequencing. The maximum enzyme production found at 37 °C at pH 7 with 1% starch. The enzyme activity was enhanced in the presence of NH4SO4 , MnSO4 and sago using colorimetric assay. Immobilization yield is found 50% by the entrapment method with 3% (w/v) Na-alginate.
"Study of amylase producing bacteria isolated from vegetable market dumping ground ", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 5, page no.2430 - 2440, May-2023, Available :http://www.ijsdr.org/papers/IJSDR2305382.pdf
Downloads:
000337212
Publication Details:
Published Paper ID: IJSDR2305382
Registration ID:206949
Published In: Volume 8 Issue 5, May-2023
DOI (Digital Object Identifier):
Page No: 2430 - 2440
Publisher: IJSDR | www.ijsdr.org
ISSN Number: 2455-2631
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