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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Troubleshooting in HPLC: A Review |
| Authors Name: | Sayali A. Bhor , Priyanka P. Atnure , Manoj S. Charde , Rita D. Chakole , Shital T. Hajare |
| Unique Id: | IJSDR2306132 |
| Published In: | Volume 8 Issue 6, June-2023 |
| Abstract: | The most effective method for many analytical procedures is chromatography. Compared to other chromatographic procedures, HPLC yields results with higher resolution and accuracy in less time. There are different types of HPLC from which reverse phase HPLC is mostly used because various components are polar in nature and easily separated. The approach to problem-solving used to identify and resolve HPLC system-related issues is known as HPLC troubleshooting. Problems with the HPLC System can come from a variety of causes. Define the issue first, then narrow down the source to identify the potential troublemaker. Troubleshooting HPLC instrumentation and separations require a fundamental understanding of how the instrument functions and how the separation works. This review is an attempt to describe overview of HPLC with its types and applications. Along with this, this article includes troubleshooting strategies and the process to overcome problems related to mobile phase, injector, detector, column and pumps. All these problems are due to pressure difference, leaks, chromatogram, baseline irregularities and smell, sight or sound related issues. |
| Keywords: | Key words: Troubleshooting, HPLC, etc. |
| Cite Article: | "Troubleshooting in HPLC: A Review", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 6, page no.924 - 933, June-2023, Available :http://www.ijsdr.org/papers/IJSDR2306132.pdf |
| Downloads: | 000337350 |
| Publication Details: | Published Paper ID: IJSDR2306132 Registration ID:207223 Published In: Volume 8 Issue 6, June-2023 DOI (Digital Object Identifier): Page No: 924 - 933 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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