Development and Validation of HPTLC Method for the Simultaneous Estimation of Loteprednol Etabonate in Drug Dosage Form.

Souravh Jha , Arti Rai , Pinky Singh , Mithun Verma , Dr V.P Gupta

Keywords: Loteprednol etabonate, HPTLC and Method Validation.

ABSTRACT A simple, accurate, precise high-performance thin-layer chromatographic method for simultaneous estimation of Loteprednol etabonate LE in ophthalmic formulation has been developed and validated. The method employed TLC aluminium plates precoated with silica gel 60F254 as stationary phase. The solvent system consisted of methanol: ethylacetate: triethylamine (7:3:0.2v/v/v). Densitometric analysis was carried out at 272nm for LE. The system was found to give compact spots for LE at Rf value of 0.70 respectively. The linear regression analysis data showed a good linear relationship in the concentration range of 1-5µg/spot for LE. The % recovery was found to be 99-102% for LE. The correlation coefficient was found to be 0.999 for LE. The % RSD values indicated that the proposed method was precise. The specificity of the method was ascertained by peak purity profiling studies and the developed method was specific. The method has been successfully applied in the analysis of combined dosage form.

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"Development and Validation of HPTLC Method for the Simultaneous Estimation of Loteprednol Etabonate in Drug Dosage Form.", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.8, Issue 9, page no.912 - 914, September-2023, Available :https://ijsdr.org/papers/IJSDR2309131.pdf

Volume 8 Issue 9, September-2023

Pages : 912 - 914

Paper Reg. ID: IJSDR_208679

Published Paper Id: IJSDR2309131

Downloads: 000347240

Research Area: Pharmacy

Country: Bhopal, Madhya Pradesh, India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2309131

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2309131

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

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