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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | Isolation and identification of PHB producing Microorganism from different soil samples |
| Authors Name: | Vidhya.N , Shruti Benny , Anushapriya.P , Sivaranjani.S |
| Unique Id: | IJSDR2312012 |
| Published In: | Volume 8 Issue 12, December-2023 |
| Abstract: | PHB is a unique intracellular polymer that accumulates under stress conditions and in the presence of an increased carbon source. Synthetic plastics, which bring many disadvantages, especially the environmental damage, are being considered and replaced by bioplastics. Bioplastics like PHB are considered to be one of the best alternatives due to their efficient biodegradability and several other effective properties. PHB-producing microorganisms were isolated by collecting soils from three different areas to compare the production of PHB in these soil samples. The isolates obtained were cultured on Minimal Davis Agar and then primary screening was performed using by Sudan Black staining. PHB production was confirmed by performing FTIR and GCMS analysis. The FTIR result showed that only organisms collected from organic soil samples showed the presence of PHB exhibiting the bands representing C=O, C-O, CH3, CH2, C-H and C-C stretches. The GC-MS spectrum of methanolized PHB was confirmed by a distinct peak corresponding to 2-oxetanone, 4-methylene, also known as 2-butynoic acid |
| Keywords: | Biodegradable PHB, Sudan Black Staining, FTIR, GC-MS Analysis |
| Cite Article: | "Isolation and identification of PHB producing Microorganism from different soil samples", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 12, page no.90 - 96, December-2023, Available :http://www.ijsdr.org/papers/IJSDR2312012.pdf |
| Downloads: | 000338719 |
| Publication Details: | Published Paper ID: IJSDR2312012 Registration ID:209156 Published In: Volume 8 Issue 12, December-2023 DOI (Digital Object Identifier): Page No: 90 - 96 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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