Chromatography latest techniques
D.Rama Brahma Reddy
, K.Malleswari , Mallela Bhavani
Pressure Liquid Chromatography, Chromatography, Method validation, Method development.
High-Performance Liquid Chromatography (HPLC) is a type of column chromatography that is commonly used in biochemistry and analysis to separate, identify, and quantify active chemicals. HPLC is the most often used separation technology for detecting, separating, and quantifying the drug. HPLC technique development and validation serve critical roles in novel drug discovery, development, and manufacturing, as well as a variety of other human and animal investigations. This review discusses the many processes involved in developing and validating an HPLC technique. The creation of an HPLC technique is influenced by the chemical structure of the molecules, the synthetic pathway, solubility, polarity, pH and pKa values, and the activity of functional groups, among other factors. Accuracy, accuracy, specificity, linearity, range, limit of detection, the limit of quantification, robustness, and system suitability testing are all included in the validation of an HPLC technique according to ICH Guidelines.
"Chromatography latest techniques", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.9, Issue 2, page no.176 - 183, February-2024, Available :https://ijsdr.org/papers/IJSDR2402026.pdf
Volume 9
Issue 2,
February-2024
Pages : 176 - 183
Paper Reg. ID: IJSDR_209995
Published Paper Id: IJSDR2402026
Downloads: 000347234
Research Area: Pharmacy
Country: -, -, India
ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
Publisher: IJSDR(IJ Publication) Janvi Wave
