ISOLATION AND IDENTIFICATION OF BACTERIA FROM GIANT FRESHWATER PRAWN Macrobrachium rosenbergii (De man ,1879)

S.Sai kumar , Dharavath Ram Kumar , Dr.t.jagadeeshwara chari

Malla Sagar Reservoir , Siddipet ,Telangana ,Latitude , Longitude , Giant freshwater prawn , Bacteria , Sample , Gut , Nutrient Agar Media , Pure Culture , Sub Culture , Gram Staining.

The Giant freshwater prawn Macrobrachium rosenbergii commonly called as scampi , farmed crustacean , species . we used in the study aim to identify the type of bacteria and stain present in gut of Giant freshwater prawn M.rosenbergii . A sample were collected from the Mallana Sagar Reservoir the Latitude 17.964° and Longitude 78.7472°are approximately located in Siddipet , dist. of Telangana state . This sample should be identified the selective species and also observed morphological, physical characteristics of selective species and then dissected and extracted the Gut sample from Giant freshwater prawn M.rosenbergii . Prepared Nutrient Agar media and sample taken to serial dilution and incubated the Pure culture media . after 24 hours in next day . we observed the bacterial about 30 colonies in sample 10th , then again prepared Nutrient Agar and incubated sub- culture , after 24 hours next day prepared bacteria smear, on slide and observed bacteria stain. This abstract examines Gram - positive bacteria were retained the crystal violet and appeared Purple and Blue colour , Gram - negative bacteria were not retain crystal violet but are count stained with safranin appeared Pink and red colour , both bacteria can exhibit various shapes cocci- round ,bacilli – rod , vibrio – comma, coccobacilli – short oval rods etc. The range of Gram – positive and Gram – negative bacteria observed and identified among the isolates 60% were Gram – positive bacteria and 40% were Gram – negative bacteria . Rod shaped bacteria Bacilli , Escherichia coli, Bacillus , lactobacillus ssp , salmonella ssp ,pseudomonas aeruginosa and comma shaped bacteria vibrio, Round or spherical shaped bacteria cocci , Staphylococcus, aureus ,streptococcus , enterococcus, micrococcus etc , these types of bacteria associated in gut of Giant freshwater prawn Macrobrachium rosenbergii.

Published Paper   E-Certificate

"ISOLATION AND IDENTIFICATION OF BACTERIA FROM GIANT FRESHWATER PRAWN Macrobrachium rosenbergii (De man ,1879)", IJSDR - International Journal of Scientific Development and Research (www.IJSDR.org), ISSN:2455-2631, Vol.10, Issue 9, page no.a672-a695, September-2025, Available :https://ijsdr.org/papers/IJSDR2509082.pdf

Volume 10 Issue 9, September-2025

Pages : a672-a695

Paper Reg. ID: IJSDR_304908

Published Paper Id: IJSDR2509082

Downloads: 00076

Research Area: Life Sciences All

Country: Siddipet , Telangana , India

Published Paper PDF: https://ijsdr.org/papers/IJSDR2509082

Published Paper URL: https://ijsdr.org/viewpaperforall?paper=IJSDR2509082

ISSN: 2455-2631 | IMPACT FACTOR: 9.15 Calculated By Google Scholar | ESTD YEAR: 2016

An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 9.15 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator

Publisher: IJSDR(IJ Publication) Janvi Wave

This work is licensed under a Creative Commons Attribution 4.0 International License and The Open Definition