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INTERNATIONAL JOURNAL OF SCIENTIFIC DEVELOPMENT AND RESEARCH International Peer Reviewed & Refereed Journals, Open Access Journal ISSN Approved Journal No: 2455-2631 | Impact factor: 8.15 | ESTD Year: 2016 open access , Peer-reviewed, and Refereed Journals, Impact factor 8.15 |
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| Paper Title: | A study on Evaluation of Anticancer compounds Behaviour of (E) -2- (3- Nitrobenzylidene) Hydrazinecarbothioamide RNA synthesis |
| Authors Name: | Dr.A.Geetha , Dr.C.Jeevarathinam , Dr.G.V.Pandian |
| Unique Id: | IJSDR2311050 |
| Published In: | Volume 8 Issue 11, November-2023 |
| Abstract: | Lung cancer is one of the primary causative agents of destruction among all diseases. More than 80% of lung cancer instance are non-small cell lung cancer (NSCLC) cases remaining 20% of lung malignant neoplastic disease are tiny cell lung cancer (SCLC). In operation of potential therapeutics for cancer, we represented herein the synthesis, characterization and in vitro anticancer activity of Meta-Nitrobenzaldehyde thiosemicarbazone (NBTSC). Meta-Nitrobenzaldehyde thiosemicarbazone is an gripping case of organic crystal. It has been mature by slow evaporation solution growth technique (SESGT) using wood alcohol as solvent. The MTT assay was practical to determine the IC50 values on A549 lung cancer cell line by NBTSC. Features of apoptosis were discovered by AO/EB and Hoechst staining. The caspase activity, Bcl-2 family, p21 and p53 proteins were calculated by RT-PCR and Western Blot analysis. The NBTSC significantly inhibited the growth of A549 cells and induced apoptosis. The NBTSC was the most potent on A549 cell line with the IC50 value of 50.50% cell viability 6µg/mL. The induction of apoptosis was overserved by chromosomal condensation, cell shrinkage, membrane blebbing and nuclear disintegration. The answers showed that NBTSC induced apoptosis were associated with an upregulation of proapoptotic Bax and down regulation of Bcl2, intemperance of mitochondrial membrane potential and activation of caspase-3. In addition to decreased mitochondrial membrane potential and overproduction of ROS demonstrated the involvement of apoptosis. Mechanistic studies further revealed that NBTSC caused the growth of the intracellular ROS, consequently aggravated the growth in p21and p53 expression and led to upregulation of Bax, depolarization of mitochondrial membrane potential and caspases cascade (caspase-3/8 and-9). The increase of p53 level by NBTSC results in the inhibition of Ribonucleotide reductase (RR) enzymes and induce programmed cell death. In this survey impart that NBTSC induces apoptosis through ROS-dependent and induce a potential healing effect for lung cancer. |
| Keywords: | Solution Growth, Slow Evaporation Technique, Spectral Characterization, Microbial Activities, Anticancer Activity, Lung Cancer. |
| Cite Article: | "A study on Evaluation of Anticancer compounds Behaviour of (E) -2- (3- Nitrobenzylidene) Hydrazinecarbothioamide RNA synthesis", International Journal of Science & Engineering Development Research (www.ijsdr.org), ISSN:2455-2631, Vol.8, Issue 11, page no.346 - 358, November-2023, Available :http://www.ijsdr.org/papers/IJSDR2311050.pdf |
| Downloads: | 000338719 |
| Publication Details: | Published Paper ID: IJSDR2311050 Registration ID:209159 Published In: Volume 8 Issue 11, November-2023 DOI (Digital Object Identifier): Page No: 346 - 358 Publisher: IJSDR | www.ijsdr.org ISSN Number: 2455-2631 |
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